Logo


V-Store


ABOUT

· Safe supplements
· Banned supplements
· Nutrition in healing
· Nutrition against disease
· Healthy catering
· Food-based guidelines
· The coral calcium scam
· What do B vitamins do
· The importance of protein
· Natural enzymes
· Nutrients in vegetables
· Nutritional supplements
· Vitamin chart
· Lose weight tips
· Calorie chart
· Low calorie recipes
· Mood disorders
· Hospital mistakes
· Hospital mishaps
· Mishaps in ICUs
· Bedsores from excessive pressure
· Wound treatment



green tea
Herba Green Tea

 
 
Liquid vitamins
Liquid vitamins



Home | Nutrition | Supplementation | Dieting | Health | Fitness | Products      
kitchenware


Biological activities of Greek bee-honey

As a part of a systematic research on the chemical composition of Greek bee-honey and, as it has been observed, an increasing interest in the use of honey in traditional Mediterranean food, in this study we report the chemical analyses and biological activities of 49 Greek honey samples produced in different regions of Greece.

All the samples were extracted with CH²Cl² and BuOH, and several chemical compounds have been isolated and identified from them. Among these: the triol E-4-(1',2',4'-Trihydroxy-2',6',6'-trimethylcyclohexyl)-but-3-en-2-one which have been shown to be unique to samples with the higher percentages from Thymus sp., the rare natural compound 3-hydroxy-4-phelyl-2-butanone just in three of our studied honey samples. Besides, the extracts as well as pure isolated compounds were assayed for their antimicrobial and antioxidative activities. The antimicrobial activities of all studied samples and of pure isolated compounds were determined, using the diffusion-dilution techniques, by measuring the MIC of them against the Gram-positive bacteria: S.aureus and S.epidermidis, and the Gram negative: P.aeruginosa, E.coli, E.cloacae, K.pneumoniae, the oral pathogens S.mutans and S.viridans, the pathogenic fungi: Candida albicans, C. tropicalis and C. glabrata as well as against the food pathogenic Listeria monocytogenes. All strains were standard of American Type Culture Collection. Standard antibiotics were used in order to control the sensitivity of the tested bacteria and fungi.
 
All the tested bee-honeys samples exhibited moderate to strong activities against the assayed microorganisms. The isolated triol and 8-hydroxy linalool showed very strong activities (MIC values ranges from 0.04-0.57 mg/ml), while the isolated butanone appeared to be less active. The samples have also been tested for their ability to protect cellular DNA from H²O²-induced single strand breaks in a T-lemphocytic cell-line (Jurkat cells). Formation of single strand breaks was estimated by using the highly sensitive methodology of comet assay (single cell gel electrophoresis). The results obtained from these experiments indicated that honey from Abies trees of Karpenissi and Vitina, orange trees of Lakonia and from Pinus of the island of EVia exhibited the best protective activities. Bioactivity-guided fractionation and isolation of the active compounds in these samples is in progress.
 
Acknowledgments
This study was supported by the General Secretariat of Research and Technology of Greece (PAVET project) by Attiki, as well as for PENED project, and "Korres" Natural Products, S.A.


E. Melliou, I. B. Chinou, University of Athens, Athens, Greece
M. Melidou, D. Galaris, University of Ioannina Medical SChool, Greece
S. Karabournioti, "Attiki" Bee-Culturing Company, Greece

 
 

Holidays in Greece in
the Greek islands


 


SECTION : MedDiet
  MENU  

· Mediterranean diet
· Evolutionary nutrition
· Past plant production
· Cyprus nutritional habits
· History of almonds
· History of chestnuts
· Malaysian diet
· Hungarian diet
· Mediterranean lifestyle
· Childhood obesity
· Polish diet
· Intake of vegetables
· Influence children
· Back to our nutrition
· Therapeutic diet
· MeDiet industrialization
· Butter replaced olive oil!
· Medi-Rivage intervention
· EPIC-Elderly study
· The SUN study
· Greek grapes
· Wine drinking in Greece
· Argentine wines
· Meat safety
· Reducing blood pressure
· Purified sardine protein
· The Cretan diet
· Greek traditional cheese
· Extra virgin Greek olive oil
· Animal products
· The Greek diet
· Reduced LDL oxidation
· The Calabria forum
· Fruits and vegetables
· Postprandial lipemia
· The Attica Study
· Greek bee honey
· Dietary d-limonene
· Olives as antioxidants
· Algerian diet type
· Culinary heritage
· Dietary questionnaire
· Obesity in Morocco
· Nutrition in Jordan
· Dyslipidemia
· Moderating oxidation
· German diet
· Cretan sample
· Overweight tendency
· Comparing 7 countries
· Blood pressure
· Mediterranean products
· Portuguese diet
· Australian diet
· Control of hypertension
· Animal welfare risks
· Dry cured hams


border line



Privacy policy - Terms of use - Contact - Site map - Links / Submit
The statements in the A-Nutritional-Supplements.com website represent the opinions of the authors.
They have not been evaluated by the Food and Drug Administration.
Copyright 2004-2014 A-Nutritional-Supplements.com